APEX-05: TOAST III
Even though we are using a different piece of growth hardware to the previous BRIC experiments, our experiment schedule is very similar. The launch is planned for December 12th, with berthing of the Dragon at the ISS 3 days later.
- Day -3 – Planting of seeds onto Phytagel in our lab in the SSPF
- Day -2 – Handover to cold stowage team for packing and loading into Dragon
- Day 0 – Launch
- Day 3 – Dragon Berthing, with ISS
- Day 7 – installation in Veggie
- Day 7 -15 Growth in Veggie
- Day 15 – Harvest plants to Kennedy Fixation tubes, chemical fixation of primary experimentation plants, Insertion of LMM plates into Veggie to start grow out for LMM experiment
- Day 16 – Freezing of primary sample KFTs
- Day 23 – Installation of plate into LMM for microscopy
- Day 24 – Harvest of LMM plate into KFT for chemical fixation
- Day 25 – Freezing of LMM sample KFTs
- Day xx – Dragon capsule returns to Earth
- Day xx – De-integration and shipment of samples from Kennedy Space Center to UW-Madison
The Big Picture
- November 2013– NASA research announcement (NRA) request for proposals.
- March 2014– Deadline for submission of proposal to NASA.
- July 2014– Notification of funding from NASA for APEX-05.
- September 2014– NASA grant begins. Start of making plant lines needed for the experiment.
- September 2015– First Gilroy Lab team visit to the Space Station Processing Facility (SSPF) lab at KSC in Florida for start of the TOAST-III Science Verification Test (SVT).
- October 2015– Return to SSPF lab (Sarah only) to process and ship SVT plants to UW-Madison.
- November 2015– Analyze the TOAST-III SVT results at UW-Madison.
- May 2016– Second Gilroy Lab team visit to KSC’s SSPF lab for start of the Experiment Verification Test (EVT)
- June 2016 Return to SSPF lab (Sarah only) to process and ship EVT plants to our lab at UW-Madison.
- July 2016– Analyze the TOAST-III EVT results at UW-Madison.
- February 2017 – Flight Readiness Review – go for launch
- September-November 2017– Launch dates pushed back: September 13 to November 1 to December 4
- November 2017– Gilroy Lab team travels to NASA Glenn on Nov 27 to test Light Microscopy Module (LMM) protocols.
- November 2017– Gilroy Lab team travels on to Florida and KSC’s SSPF lab Nov 28 to prepare for Dec 4 launch;
- December 2017– Launch date pushed back to December 8, then to December 12.
- January 2018– Sample return and de-integration at KSC.
BRIC-19: TOAST II & GeneLAB
This was the schedule for the SVT, PVT, and flight for BRIC-17 TOAST which flew to the ISS in March of 2013 on SpaceX CRS-2. The same schedule will be used for our BRIC-19 TOAST II & GeneLAB experiments, which will fly on SpaceX CRS-4 (SpX-4) mission. The launch for SpX-4 is planned for September 19, 2014; berthing of the Dragon capsule to the ISS will happen 2 days later on September 21, 2014.
- Day -4 – Planting of seeds onto phytagel in our lab in the SSPF.
- Day -3 – Manifold and valve assembly for the PDFU.
- Day -2 – Integration: “Build-up” of flight PDFU including science, PDFU leak testing, and canister assembly.
- Day 0 – Launch.
- Day 2 through 8 – Growth of seedlings in microgravity on the ISS.
- Day 8 – Actuation of the flight BRIC canisters (fixation liquid) by an astronaut.
- Day 9 – Freeze canisters.
- Day xx – Dragon capsule landing.
- Day xx – De-integration, sample processing and shipment to University of Wisconsin – Madison.
BRIC-17: TOAST I
Here’s a look at the timeline of the Gilroy Lab’s NASA funded BRIC-17 Test Of Arabidopsis Space Transcriptome (TOAST) project, the first BRIC experiment our lab sent to the ISS in 2013.
The Big Picture
- September 2011 – NASA research announcement request for proposals (RFP).
- January 2012 – Deadline for submission of proposal to NASA.
- April 2012 – Notification of funding from NASA for BRIC-17.
- June 2012 – NASA grant begins.
- July 2012 – First Gilroy Lab team visit to the Space Life Science (SLS) lab at KSC in Florida for start of the TOAST Science Verification Test (SVT).
- August 2012 – Return to SLS lab (Sarah only) to process and ship SVT plants to UW-Madison.
- September 2012 – Analyse the BRIC-17 SVT results at UW-Madison.
- October 2012 – Second Gilroy Lab team visit to KSC’s SLS lab for start of the Payload Verification Test (PVT); Return to SLS lab to process and ship PVT plants to our lab at UW-Madison.
- November 2012 – Analyse the BRIC-17 TOAST PVT results at UW-Madison.
- March 2013 – Third Gilroy Lab team visit to KSC’s SLS lab for launch of SpaceX-2; BRIC-17 runs on the ISS in parallel with ground controls.
- April 2013 – Return of the BRIC-17 TOAST experiment from the ISS back to Earth; Gilroy Lab team visits the SLS lab to process and ship space-flown and Earth-grown control plants to UW-Madison.
- May 2013 to 2014 – Analyse the space-flown and Earth-grown BRIC-17 TOAST seedlings.